Overexpression of PtrMYB119, a R2R3-MYB transcription factor from Populus trichocarpa, promotes anthocyanin production in hybrid poplar.
Identifieur interne : 001740 ( Main/Exploration ); précédent : 001739; suivant : 001741Overexpression of PtrMYB119, a R2R3-MYB transcription factor from Populus trichocarpa, promotes anthocyanin production in hybrid poplar.
Auteurs : Jin-Seong Cho [Corée du Sud] ; Van Phap Nguyen [Corée du Sud] ; Hyung-Woo Jeon [Corée du Sud] ; Min-Ha Kim [Corée du Sud] ; Seok Hyun Eom [Corée du Sud] ; You Jin Lim [Corée du Sud] ; Won-Chan Kim [Corée du Sud] ; Eung-Jun Park [Corée du Sud] ; Young-Im Choi [Corée du Sud] ; Jae-Heung Ko [Corée du Sud]Source :
- Tree physiology [ 1758-4469 ] ; 2016.
Descripteurs français
- KwdFr :
- Anthocyanes (biosynthèse), Arabidopsis (MeSH), Arbres (génétique), Arbres (métabolisme), Clonage moléculaire (MeSH), Facteurs de transcription (métabolisme), Populus (génétique), Populus (métabolisme), Protéines associées à la pancréatite (MeSH), Protéines végétales (métabolisme), Végétaux génétiquement modifiés (MeSH).
- MESH :
- biosynthèse : Anthocyanes.
- génétique : Arbres, Populus.
- métabolisme : Arbres, Facteurs de transcription, Populus, Protéines végétales.
- Arabidopsis, Clonage moléculaire, Protéines associées à la pancréatite, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Anthocyanins.
- chemical , metabolism : Plant Proteins, Transcription Factors.
- genetics : Populus, Trees.
- metabolism : Populus, Trees.
- Arabidopsis, Cloning, Molecular, Pancreatitis-Associated Proteins, Plants, Genetically Modified.
Abstract
Anthocyanins are a group of colorful and bioactive natural pigments with important physiological and ecological functions in plants. We found an MYB transcription factor (PtrMYB119) from Populus trichocarpa that positively regulates anthocyanin production when expressed under the control of the CaMV 35S promoter in transgenic Arabidopsis Amino acid sequence analysis revealed that PtrMYB119 is highly homologous to Arabidopsis PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), a well-known transcriptional activator of anthocyanin biosynthesis. Independently produced transgenic poplars overexpressing PtrMYB119 or PtrMYB120 (a paralogous gene to PtrMYB119) (i.e., 35S::PtrMYB119 and 35S::PtrMYB120, respectively) showed elevated accumulation of anthocyanins in the whole plants, including leaf, stem and even root tissues. Using a reverse-phase high-performance liquid chromatography, we confirmed that the majority of the accumulated anthocyanin in our transgenic poplar is cyanidin-3-O-glucoside. Gene expression analyses revealed that most of the genes involved in the anthocyanin biosynthetic pathway were highly upregulated in 35S::PtrMYB119 poplars compared with the nontransformed control poplar. Among these genes, expression of PtrCHS1 (Chalcone Synthase1) and PtrANS2 (Anthocyanin Synthase2), which catalyze the initial and last steps of anthocyanin biosynthesis, respectively, was upregulated by up to 350-fold. Subsequent transient activation assays confirmed that PtrMYB119 activated the transcription of both PtrCHS1 and PtrANS2 Interestingly, expression of MYB182, a repressor of both anthocyanin and proanthocyanidin (PA) biosynthesis, was largely suppressed in 35S::PtrMYB119 poplars, while expression of MYB134, an activator of PA biosynthesis, was not changed significantly. More interestingly, high-level accumulation of anthocyanins in 35S::PtrMYB119 poplars did not have an adverse effect on plant growth. Taken together, our results demonstrate that PtrMYB119 and PtrMYB120 function as transcriptional activators of anthocyanin accumulation in both Arabidopsis and poplar.
DOI: 10.1093/treephys/tpw046
PubMed: 27259636
Affiliations:
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Korea Forest Research Institute, Suwon 16631, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631</wicri:regionArea><wicri:noRegion>Suwon 16631</wicri:noRegion></affiliation></author><author><name sortKey="Nguyen, Van Phap" sort="Nguyen, Van Phap" uniqKey="Nguyen V" first="Van Phap" last="Nguyen">Van Phap Nguyen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104</wicri:regionArea><wicri:noRegion>Yongin 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Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104</wicri:regionArea><wicri:noRegion>Yongin 17104</wicri:noRegion></affiliation></author><author><name sortKey="Eom, Seok Hyun" sort="Eom, Seok Hyun" uniqKey="Eom S" first="Seok Hyun" last="Eom">Seok Hyun Eom</name><affiliation wicri:level="1"><nlm:affiliation>Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104</wicri:regionArea><wicri:noRegion>Yongin 17104</wicri:noRegion></affiliation></author><author><name sortKey="Lim, You Jin" sort="Lim, You Jin" uniqKey="Lim Y" first="You Jin" last="Lim">You Jin Lim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104</wicri:regionArea><wicri:noRegion>Yongin 17104</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Won Chan" sort="Kim, Won Chan" uniqKey="Kim W" first="Won-Chan" last="Kim">Won-Chan Kim</name><affiliation wicri:level="1"><nlm:affiliation>School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Park, Eung Jun" sort="Park, Eung Jun" uniqKey="Park E" first="Eung-Jun" last="Park">Eung-Jun Park</name><affiliation wicri:level="1"><nlm:affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631</wicri:regionArea><wicri:noRegion>Suwon 16631</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Young Im" sort="Choi, Young Im" uniqKey="Choi Y" first="Young-Im" last="Choi">Young-Im Choi</name><affiliation wicri:level="1"><nlm:affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea yichoi99@korea.kr jhko@khu.ac.kr.</nlm:affiliation><country wicri:rule="url">Corée du Sud</country><wicri:regionArea>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631</wicri:regionArea><wicri:noRegion>Suwon 16631</wicri:noRegion></affiliation></author><author><name sortKey="Ko, Jae Heung" sort="Ko, Jae Heung" uniqKey="Ko J" first="Jae-Heung" last="Ko">Jae-Heung Ko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea yichoi99@korea.kr jhko@khu.ac.kr.</nlm:affiliation><country wicri:rule="url">Corée du Sud</country><wicri:regionArea>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104</wicri:regionArea><wicri:noRegion>Yongin 17104</wicri:noRegion></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anthocyanins (biosynthesis)</term><term>Arabidopsis (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Pancreatitis-Associated Proteins (MeSH)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Transcription Factors (metabolism)</term><term>Trees (genetics)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anthocyanes (biosynthèse)</term><term>Arabidopsis (MeSH)</term><term>Arbres (génétique)</term><term>Arbres (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Facteurs de transcription (métabolisme)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines associées à la pancréatite (MeSH)</term><term>Protéines végétales (métabolisme)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Anthocyanins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anthocyanes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arbres</term><term>Facteurs de transcription</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Arabidopsis</term><term>Cloning, Molecular</term><term>Pancreatitis-Associated Proteins</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arabidopsis</term><term>Clonage moléculaire</term><term>Protéines associées à la pancréatite</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Anthocyanins are a group of colorful and bioactive natural pigments with important physiological and ecological functions in plants. We found an MYB transcription factor (PtrMYB119) from Populus trichocarpa that positively regulates anthocyanin production when expressed under the control of the CaMV 35S promoter in transgenic Arabidopsis Amino acid sequence analysis revealed that PtrMYB119 is highly homologous to Arabidopsis PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), a well-known transcriptional activator of anthocyanin biosynthesis. Independently produced transgenic poplars overexpressing PtrMYB119 or PtrMYB120 (a paralogous gene to PtrMYB119) (i.e., 35S::PtrMYB119 and 35S::PtrMYB120, respectively) showed elevated accumulation of anthocyanins in the whole plants, including leaf, stem and even root tissues. Using a reverse-phase high-performance liquid chromatography, we confirmed that the majority of the accumulated anthocyanin in our transgenic poplar is cyanidin-3-O-glucoside. Gene expression analyses revealed that most of the genes involved in the anthocyanin biosynthetic pathway were highly upregulated in 35S::PtrMYB119 poplars compared with the nontransformed control poplar. Among these genes, expression of PtrCHS1 (Chalcone Synthase1) and PtrANS2 (Anthocyanin Synthase2), which catalyze the initial and last steps of anthocyanin biosynthesis, respectively, was upregulated by up to 350-fold. Subsequent transient activation assays confirmed that PtrMYB119 activated the transcription of both PtrCHS1 and PtrANS2 Interestingly, expression of MYB182, a repressor of both anthocyanin and proanthocyanidin (PA) biosynthesis, was largely suppressed in 35S::PtrMYB119 poplars, while expression of MYB134, an activator of PA biosynthesis, was not changed significantly. More interestingly, high-level accumulation of anthocyanins in 35S::PtrMYB119 poplars did not have an adverse effect on plant growth. Taken together, our results demonstrate that PtrMYB119 and PtrMYB120 function as transcriptional activators of anthocyanin accumulation in both Arabidopsis and poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27259636</PMID><DateCompleted><Year>2017</Year><Month>08</Month><Day>30</Day></DateCompleted><DateRevised><Year>2017</Year><Month>12</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>36</Volume><Issue>9</Issue><PubDate><Year>2016</Year><Month>09</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Overexpression of PtrMYB119, a R2R3-MYB transcription factor from Populus trichocarpa, promotes anthocyanin production in hybrid poplar.</ArticleTitle><Pagination><MedlinePgn>1162-76</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpw046</ELocationID><Abstract><AbstractText>Anthocyanins are a group of colorful and bioactive natural pigments with important physiological and ecological functions in plants. We found an MYB transcription factor (PtrMYB119) from Populus trichocarpa that positively regulates anthocyanin production when expressed under the control of the CaMV 35S promoter in transgenic Arabidopsis Amino acid sequence analysis revealed that PtrMYB119 is highly homologous to Arabidopsis PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), a well-known transcriptional activator of anthocyanin biosynthesis. Independently produced transgenic poplars overexpressing PtrMYB119 or PtrMYB120 (a paralogous gene to PtrMYB119) (i.e., 35S::PtrMYB119 and 35S::PtrMYB120, respectively) showed elevated accumulation of anthocyanins in the whole plants, including leaf, stem and even root tissues. Using a reverse-phase high-performance liquid chromatography, we confirmed that the majority of the accumulated anthocyanin in our transgenic poplar is cyanidin-3-O-glucoside. Gene expression analyses revealed that most of the genes involved in the anthocyanin biosynthetic pathway were highly upregulated in 35S::PtrMYB119 poplars compared with the nontransformed control poplar. Among these genes, expression of PtrCHS1 (Chalcone Synthase1) and PtrANS2 (Anthocyanin Synthase2), which catalyze the initial and last steps of anthocyanin biosynthesis, respectively, was upregulated by up to 350-fold. Subsequent transient activation assays confirmed that PtrMYB119 activated the transcription of both PtrCHS1 and PtrANS2 Interestingly, expression of MYB182, a repressor of both anthocyanin and proanthocyanidin (PA) biosynthesis, was largely suppressed in 35S::PtrMYB119 poplars, while expression of MYB134, an activator of PA biosynthesis, was not changed significantly. More interestingly, high-level accumulation of anthocyanins in 35S::PtrMYB119 poplars did not have an adverse effect on plant growth. Taken together, our results demonstrate that PtrMYB119 and PtrMYB120 function as transcriptional activators of anthocyanin accumulation in both Arabidopsis and poplar.</AbstractText><CopyrightInformation>© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Jin-Seong</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Van Phap</ForeName><Initials>VP</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jeon</LastName><ForeName>Hyung-Woo</ForeName><Initials>HW</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Min-Ha</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eom</LastName><ForeName>Seok Hyun</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>You Jin</ForeName><Initials>YJ</Initials><AffiliationInfo><Affiliation>Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Won-Chan</ForeName><Initials>WC</Initials><AffiliationInfo><Affiliation>School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Eung-Jun</ForeName><Initials>EJ</Initials><AffiliationInfo><Affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Young-Im</ForeName><Initials>YI</Initials><AffiliationInfo><Affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea yichoi99@korea.kr jhko@khu.ac.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ko</LastName><ForeName>Jae-Heung</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 446-701, Yongin 17104, Republic of Korea yichoi99@korea.kr jhko@khu.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000872">Anthocyanins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000073718">Pancreatitis-Associated Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000612141">REG3A protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000872" MajorTopicYN="N">Anthocyanins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000073718" MajorTopicYN="N">Pancreatitis-Associated Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">pigment</Keyword><Keyword MajorTopicYN="Y">transcriptional activator</Keyword><Keyword MajorTopicYN="Y">transgenic poplar</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>10</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>04</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>8</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27259636</ArticleId><ArticleId IdType="pii">tpw046</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpw046</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Cho, Jin Seong" sort="Cho, Jin Seong" uniqKey="Cho J" first="Jin-Seong" last="Cho">Jin-Seong Cho</name></noRegion><name sortKey="Choi, Young Im" sort="Choi, Young Im" uniqKey="Choi Y" first="Young-Im" last="Choi">Young-Im Choi</name><name sortKey="Eom, Seok Hyun" sort="Eom, Seok Hyun" uniqKey="Eom S" first="Seok Hyun" last="Eom">Seok Hyun Eom</name><name sortKey="Jeon, Hyung Woo" sort="Jeon, Hyung Woo" uniqKey="Jeon H" first="Hyung-Woo" last="Jeon">Hyung-Woo Jeon</name><name sortKey="Kim, Min Ha" sort="Kim, Min Ha" uniqKey="Kim M" first="Min-Ha" last="Kim">Min-Ha Kim</name><name sortKey="Kim, Won Chan" sort="Kim, Won Chan" uniqKey="Kim W" first="Won-Chan" last="Kim">Won-Chan Kim</name><name sortKey="Ko, Jae Heung" sort="Ko, Jae Heung" uniqKey="Ko J" first="Jae-Heung" last="Ko">Jae-Heung Ko</name><name sortKey="Lim, You Jin" sort="Lim, You Jin" uniqKey="Lim Y" first="You Jin" last="Lim">You Jin Lim</name><name sortKey="Nguyen, Van Phap" sort="Nguyen, Van Phap" uniqKey="Nguyen V" first="Van Phap" last="Nguyen">Van Phap Nguyen</name><name sortKey="Park, Eung Jun" sort="Park, Eung Jun" uniqKey="Park E" first="Eung-Jun" last="Park">Eung-Jun Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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